Optimization of HEK293T suspension cultivation with a DOE-approach in the ambr® 15 micro bioreactor
Time: 15.00-16.00 (CET)
Sartorius Stedim Data Analytics (SSDA) invites to a webinar on a MODDE/DOE use case in the ambr® 15 micro bioreactor.
Viral vectors such as adenoviruses, adeno-associated viruses and retroviruses are effective delivery systems for genetic material used in cell and gene therapies. Especially lentiviruses are used for example for the transfer of genetic information for novel cellular immunotherapy like CAR-T cell therapy.
HEK293T cells are a workhorse cell line for lentiviral vector production for both cell and gene therapy applications. A significant challenge for the cell and gene therapy industry is to develop a HEK293T suspension cell culture process that is well characterized and can be scaled-up for production whilst ensuring clinical and commercial success.
In the study presented ambr® 15 was used for the optimization of the HEK293T culture in suspension. Optimal stirring speed, DO and pH value was identified by performing a DoE study by using the MODDE® software for experimental planning. Viable Cell Count (VCC), viability and generation time have been monitored and compared to standard shake flask culture.
This webinar is presented by Dr. Franziska Bollmann, who is a virus scientist in the Regenerative Medicine R&D Department at Sartorius Stedim Biotech, Göttingen, Germany. She is a studied biochemist with experience in lentivirus up- and downstream processing and assay development.
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